Table of Contents

• A. About the Editor
• B. Preface
• 1. Toward Automated Breast Histopathology Using Mid-IR Spectroscopic Imaging
• 2. Synchrotron-Based FTIR Spectromicroscopy and Imaging of Single Algal Cells and Cartilage
• 3. Preparation of Tissues and Cells for Infrared and Raman Spectroscopy and Imaging
• 4. Evanescent Wave Imaging
• 5. sFTIR, Raman, and SERS Imaging of Fungal Cells
• 6. Widefield Raman Imaging of Cells and Tissues
• 7. Resonance Raman Imaging and Quantification of Carotenoid Antioxidants in the Human Retina and Skin
• 8. Raman Microscopy for Biomedical Applications: Toward an Efficient Diagnosis of Tissues, Cells, and Bacteria
• 9. The Current State of Raman Imaging in Clinical Application
• 10. Vibrational Spectroscopic Imaging of Microscopic Stress Patterns in Biomedical Materials
• 11. Tissue Imaging with Coherent Anti-Stokes Raman Scattering Microscopy

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Expanded Table of Contents

• A. About the Editor
• B. Preface
• 1. Toward Automated Breast Histopathology Using Mid-IR Spectroscopic Imaging
  • Introduction
  • Materials and Methods
  • Conclusions
• 2. Synchrotron-Based FTIR Spectromicroscopy and Imaging of Single Algal Cells and Cartilage
  • Introduction
  • IR Environmental Imaging
  • Flow Cell for In Vivo IR Microspectroscopy of Biological Samples
  • Biomedical Application: Calcium-Containing Crystals in Arthritic Cartilage
  • Future Directions: In Vivo Kinetics of Pathological Mineralization and Phytoplankton Adaptation
  • Acknowledgments
• 3. Preparation of Tissues and Cells for Infrared and Raman Spectroscopy and Imaging
  • Introduction
  • Tissue Preparation
  • Cell Preparation
  • Summary
  • Acknowledgments
  • References
• 4. Evanescent Wave Imaging
  • Introduction
  • Theoretical Considerations
  • Historical Development
  • Experimental Implementation
  • Benefits of ATR Microspectroscopic Imaging for Biological Sections
  • Macro ATR Imaging
  • ATR Microspectroscopic Raman Imaging
  • Conclusions
• 5. sFTIR, Raman, and SERS Imaging of Fungal Cells
  • Introduction
  • Introduction to Fungi
  • Vibrational Spectroscopy
  • sFTIR Spectra of Fungi
  • Raman Spectroscopy of Fungi
  • SERS Discovery and Development
  • Conclusions: Lessons Learned, Caveats, Challenges, Promise
  • Acknowledgments
• 6. Widefield Raman Imaging of Cells and Tissues
  • Introduction
  • Generation of Raman Images
  • Raman Imaging of Cells and Tissues
  • Background and Image Preprocessing Steps for Widefield Raman Images
  • Chemometric Analysis of Widefield Raman Images
  • Chemometrics in the Analysis of Non-Widefield Raman Images
  • Conclusions
• 7. Resonance Raman Imaging and Quantification of Carotenoid Antioxidants in the Human Retina and Skin
  • Introduction
  • Optical Properties and Resonance Raman Scattering of Carotenoids
  • Spatially Integrated Resonance Raman Measurements of Macular Pigment
  • Spatially Resolved Resonance Raman Imaging of Macular Pigment—Methodology and Validation Experiments
  • Spatially Resolved Resonance Raman Imaging of Macular Pigment in Human Subjects
  • Raman Detection of Carotenoids in Living Human Skin
  • Conclusions
• 8. Raman Microscopy for Biomedical Applications: Toward an Efficient Diagnosis of Tissues, Cells, and Bacteria
  • Introduction
  • Raman Imaging of Tissue
  • Raman Imaging of Cells
  • Red Blood Cells
  • Raman Spectroscopy of Bacteria
  • Conclusions
  • Acknowledgments
• 9. The Current State of Raman Imaging in Clinical Application
  • Introduction
  • Instrumentation
  • Imaging Techniques
  • Data Analysis: Spectra to Image(s)
  • Raman Mapping and Imaging in Bioscience
  • Limitations and Perspectives
• 10. Vibrational Spectroscopic Imaging of Microscopic Stress Patterns in Biomedical Materials
  • Introduction
  • Principles of Raman Spectroscopy
  • Raman Effect in Biological and Synthetic Biomaterials
  • Visualization of Microscopic Stress Patterns in Biomaterials
  • Conclusions
• 11. Tissue Imaging with Coherent Anti-Stokes Raman Scattering Microscopy
  • From Spontaneous to Coherent Raman Spectroscopy
  • The Birth of CARS Microscopy
  • CARS Basics
  • CARS by the Numbers
  • CARS and the Multimodal Microscope
  • CARS in Tissues
  • CARS Biomedical Imaging
  • What Lies at the Horizon?
  • Acknowledgments